sebastian kadener lab

@skadener. Fededa J.P., Petrillo E., Gelfand M.S., Neverov A.D., Kadener S., Nogues G., Pelisch F., Baralle F.E., Muro A.F. Working under the mentorship of Dr. Beth Lawlor, April focused on understanding how hijacking of normal developmental pathways lead to heterogeneity and cell state plasticity in Ewing sarcoma. Zaffagni M, Kadener S. NEAT1 is overexpressed in Parkinson's disease substantia nigra and confers drug-inducible neuroprotection from oxidative stress. Perelman School of Medicine at the University of Pennsylvania Lerner I. Determine the nature of circadian rhythms within organisms. I am interested in the mechanisms of post transcriptional regulation of the core circadian feedback loop. de la Mata M., Alonso C.R., Kadener S., Fededa J.P., Blaustein M., Pelisch F., Cramer P., Bentley D. and Kornblihtt A.R. Molecular Neurobiology and RNA metabolism. Studies from our lab and others have documented that many signaling pathways trigger stem cells to adjust their rate of proliferation/differentiation to meet the demand for tissue regeneration. * Corresponding authors. In addition, skeletal muscles produce various myokines that influence metabolic homeostasis, lifespan, and the progression of age-related diseases and aging in non-muscle tissues. Mezan S., Feuz JD, Deplancke B. and Kadener S. PDF signaling is an integral part of the. Selected for Faculty of 1000 (Factor 3 Recommended). View 129 related references Ask authors/readers for more resources. and Mimi is determined to introduce her to cricket and Aussie footy (AFL). DIOPT has been well received by the scientific community and has been integrated into FlyBase, PomBase, MARRVEL (Model organism Aggregated Resources for Rare Variant ExpLoration) and AGR (Alliance of Genome Resources); Gene2Function integrates annotation information for orthologs of multiple species in unified interfaces; RSVP allows scientists to mine the validation and phenotype data for in vivo RNAi and sgRNA stocks; MIST (Molecular Interaction Search Tool) for mining, visualization and analysis of network data, iProteinDB for mining protein post-translational modification (PTM) data and comparison of PTM data across species and DRscDB for mining and comparison of single-cell RNA-seq data across species. We are currently using this model to identify additional factors derived from tumors that are involved in the organ wasting phenotype and to study the wasting process in peripheral tissues. I am a PhD student in the lab since 2018 after graduating from The College of Wooster. From such genetic screens we have already characterized a number of secreted factors by which organs communicate their physiological state to others: ImpL2/IGFBP, Myostatin/GDF11, Upd2/Leptin, Activin-beta, Upd3/IL6, and Pvf1/PDGF. Statuses. With this advance, we hope to enable the mosquito community to easily perform genome-wide loss- or gain-of-function screens in cells, for example to discover entry and infection mechanisms of pathogens and drugs, as well as to aid in the functional annotation of mosquito genomes. Brown S.A., Ripperger J., Kadener S., Fleury-Olela F., Vilbois F., Rosbash M. and Schibler U. Control of alternative pre-mRNA splicing by RNA Pol II elongation: faster is not always better. We also identified the first factor involved in circRNA production, the splicing factormuscleblind. Andranik Ivanov and Sebastian Meczmack (Prof. Rajewsky's . Fun in the Lab. Wittenbrink N, Ananthasubramaniam B, Mnch M, Koller B, Maier B, Weschke C, Bes F, de Zeeuw J, Nowozin C, Wahnschaffe A, Wisniewski S, Zaleska M, Bartok O, Ashwal-Fluss R, Lammert H, Herzel H, Hummel M, Kadener S, Kunz D, Kramer A. Levin-Klein R., Fraenkel S., Lichtenstein M., Matheson L., Bartok O., Nevo Y., Kadener S., Corcoran A., Cedar H., Bergman Y. Sebastian Kadener Principal Investigator M.Sc., The University of Buenos Aires, Buenos Aires, Argentina Ph.D., The University of Buenos Aires, Buenos Aires, Argentina skadener [at]brandeis [dot]edu Staff Sinead Nguyen Lab Manager I am interested in studying circular RNA formation. In the Bandopadhayay Lab, Jess is applying genomic approaches to optimize novel treatment approaches for pediatric brain tumors, and is also working to characterize the role long non-coding RNAs play in the formation of Diffuse Intrinsic Pontine Gliomas. Fun fact:In her free time, Marissa loves to go on bike rides, do yoga and walk her dog. Im working on aging-related circRNA knockdown fly behavior and physiology. B.S., University of Barcelona, Spain Ph.D., University of Leicester, UK, I am studying the circadian clock of Drosophila, B.S. Fun fact: Anna is a gardening nerd on hold, as she does not have a garden in Boston. News. Articles Cited by Public access Co-authors. For loss-of-function, the lines express one or two gRNAs targeting the coding sequence of a gene or genes. Cramer P., Srebrow A., Kadener S., Werbajh S., de la Mata M., Melen G., Nogues G. and Kornblihtt AR. Marissa received her BS in Biochemistry and Molecular Biology from UMass Amherst in 2022. The tagged endogenous proteins can then be isolated for further analysis by MS. To analyze protein-protein interactions or identify components that localize to discrete subcellular compartments, we developed tools based on APEX, an engineered ascorbate peroxidase derived from plants, and BioID, a mutant form of the biotin ligase BirA from E coli and demonstrated their use for in vivo studies. Fun fact: Dana is a huge Philadelphia sports fan and is mentally preparing to be the only Eagles fan in Boston. We use Drosophila as a model system to characterize the mechanisms underlying communication between cells, tissues, and organs. . We are particularly interested on the role of circular RNAs (circRNAs) at the molecular and neural levels as well as the mechanisms underlying circadian clocks. His main research interests include the roles of protein homeostasis, RNA metabolism, and intracellular transport in neurodegeneration. I am interested in studying circular RNA formation. While the former supposes a temperature-dependent balancing of reactions with opposite effects on circadian period, the . In addition, recently we generated the first set of animals in which specific circRNAs are downregulated. 290(2015). Alternative splicing regulates biogenesis of miRNAs located across exon-intron junctions. Wnt/Wingless, Insulin Receptor, EGFR, INR, JNK, JAK/STAT, Notch, and Hippo pathways play roles in the division and differentiation of ISCs in the context of homeostasis and/or injury. He then switched gears to study medicine while simultaneously starting his PhD work in the lab of Dr. Lena Claesson-Welsh at Uppsala University. In the past 50 years, studies using genetically tractable model systems have led to a detailed understanding of genetic mechanisms involved in the control of developmental events, as illustrated by our knowledge of patterning and morphogenesis. Transcriptional activators differ in their abilities to control alternative splicing. Stem cell fate is determined by the interplay of lineage-specific intrinsic factors and extrinsic signals, acting primarily at the transcriptional level. Metazoan MicroRNAs (2018) David P. Bartel CELL Non-canonical functions of microRNA pathway enzymes - Drosha, DGCR8, Dicer and Ago proteins S Kadener, P Cramer, G Nogus, D Cazalla, M de la Mata, JP Fededa, . Exonic circular RNAs (circRNAs) are mostly generated from exons of protein-coding genes and, in many cases, are more abundant that the linear . In the past 50 years, studies using genetically tractable model systems have led to a detailed understanding of genetic mechanisms involved in the control of developmental events, as illustrated by our knowledge of patterning and morphogenesis. and Torres H.N. Bartok O., Kyriacou C., Levine J., Sehgal A. and Kadener S. Adaptation of molecular circadian clockwork to environmental changes: a role for alternative splicing and miRNAs. Hanan M, Simchovitz A, Yayon N, Vaknine S, Cohen-Fultheim R, Karmon M, Madrer N, Rohrlich TM, Maman M, Bennett ER, Greenberg DS, Meshorer E, Levanon EY, Soreq H, Kadener S. Past, present, and future of circRNAs. Kadener S., Cramer P., Nogues G., Cazalla D., de la Mata M., Fededa J., Werbajh S., Srebrow A. and Kornblihtt A. CircRNAs expression levels are not correlated with the expression of their linear isoforms, indicating a potentially widespread layer of previously unknown gene regulation. Murugan et al. Followers. Nov, 2017. Lacadie S.A., Tardiff D.F., Kadener S. and Rosbash M. In vivo commitment to yeast cotranscriptional splicing is sensitive to transcription elongation mutants. 1, Artyomov M., Shishkin A., Kadri S., Zhu X., Gutman M., Garber M.* and Kadener S.* Defining the 5 and 3 landscape of theDrosophilatranscriptome with ExoCAGE and RNaseH-seq. de la Mata M., Alonso C.R., Kadener S., Fededa J.P., Blaustein M., Pelisch F., Cramer P., Bentley D. and Kornblihtt A.R. and Kadener S. Mathematical Model of the, Kadener S., Menet J., Sugino K., Horwich M.D., Weissbein U., Nawathean P., Vagin V., Zamore P., Nelson S. and Rosbash M. A role for miRNAs in the, Kadener S., Rodriguez J., Abruzzi K. and Rosbash M. Genome-wide identification of targets of the, Kadener S., Schoer R., Menet J. and Rosbash M. Circadian transcription contributes to core period determination in, Kadener S., Stoleru D., McDonald M., Nawathean P. and Rosbash M. , Rosbash M., Bradley S., Kadener S., Li Y., Luo W., Menet J.S., Nagoshi E., Palm K., Schoer R., Shang Y. and Tang C.H. Selected for Faculty of 1000 (Factor 6 Must Read). Transcriptional feedback and definition of the circadian pacemaker in, Kadener S., Villella A., Kula E., Palm K., Pyza E., Botas J., Hall J.C. and Rosbash M. Neurotoxic protein expression reveals connections between the circadian clock and mating behavior in. Aaron received his medical degree from Thomas Jefferson University in 2011. These lines can be used to validate results from genome-wide RNAi screens as well as to conduct low- or high-throughput genetic screens and other studies in vivo. 1, Artyomov M., Shishkin A., Kadri S., Zhu X., Gutman M., Garber M.* and Kadener S.* Defining the 5 and 3 landscape of theDrosophilatranscriptome with ExoCAGE and RNaseH-seq. Following our identification of adult Drosophila intestinal stem cells (ISCs), we used this system to characterize genes and pathways important for gut homeostasis and in particular, to analyze crosstalk among signaling pathways. She also loves sleeping in, making guacamole, and reading about art history. Cold Spring Harbor Laboratory Online. Cs. Molecular Neurobiology and RNA Metabolism Laboratory. for mining and comparison of single-cell RNA-seq data across species. For example, little is understood about how the simultaneous growth and differentiation of different tissues and organs are coordinated and how the development of different cell types and tissues is integrated within an organ. We generated a dual reporter with a destabilized fast folding GFP (dsGFP) protein and a stable RFP reporter. Tattikota SG., Rathjen T., Hausser J., Khedkar A., Kabra UD., Pandey V., Sury M., Wessels HH., Mollet IG., Eliasson L., Selbach M., Zinzen RP., Zavolan M., Kadener S, Tschp MH, Jastroch M, Friedlnder MR., Poy MN. More. Samar was awarded the Neubauer doctoral fellowship . Boston, MA. . We showed that miRNAs have a key role in providing robustness to the circadian system both during development and in adults. APEX, an engineered ascorbate peroxidase derived from plants, BioID, a mutant form of the biotin ligase BirA from. These nanobodies and NanoTags can be expressed as chromobodies that enable detecting NanoTags at the N-terminus, C-terminus, or internal site of protein of interest. Following. He is also contributing to efforts to characterize chromatin complexes as therapeutic targets for pediatric brain tumors. From 2002 to 2008, Sebastian Kadener was a postdoc working in the Michael Rosbash laboratory. For doing this, we study the circadian clock from a systemic point of view, including studies at the molecular and neural levels. She lived in Oregon and Connecticut and move to Massachusetts, where she received her BA in Biochemistry from Wellesley College (22). We believe that this is reminiscent of changes in ISC proliferation observed in animals that feed infrequently, such as the Burmese python, where ISC proliferation is triggered following a large meal as a result of gut distortion. Furthermore, dehydration of the intraluminal surface in miR-263a mutant guts increases bacterial infection, as evident by the increased expression of antimicrobial peptides. As part of his bachelors program, he joined the group of Prof. Tony Kourzarides at the University of Cambridge in 2019 as a visiting undergraduate student to investigate the role and pharmacological inhibition of histone acetyl transferases in leukemia. Studying the neuronal functions of circular RNA. Interestingly, mechanical stress caused by overfeeding flies with 10% methylcellulose, which distorts the gut, induces ISC proliferation. and Kornblihtt A.R. As miR-183, the human ortholog of miR-263a, also regulates ENaC, our findings suggest miR-183 might be used as a treatment for CF. Rybak-Wolf A., Stottmeister C., Glazar P., Jens M., Pino N., Giusti S., Hanan M., Behm M., Bartok O., Ashwal R., Herzog M., Schreyer L., Papavasileiou P., Ivanov A., Ohman M., Refojo D., Kadener S. and Rajewsky N. Circular RNAs in the mammalian brain are highly abundant, conserved, dynamically expressed, and regulated by ADAR1. Community support Reprint. Mosquito-borne diseases, including Dengue, Zika, Chikungunya and West Nile Virus, present a worldwide public health burden. The technologies we use for CRISPR screening in Drosophila cells are extensible to other cell types. High-accuracy determination of internal circadian time from a single blood sample. Pandey V., Turm H., Bekenstein U., Shifman S. and Kadener S. A new. For example, little is understood about how the simultaneous growth and differentiation of different tissues and organs are coordinated and how the development of different cell types and tissues is integrated within an organ. 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Oregon and Connecticut and move to Massachusetts, where she received her BA in Biochemistry from College! Antimicrobial peptides S.A., Tardiff D.F., Kadener S. a new nigra confers! Deplancke B. and Kadener S. a new F., Rosbash M. and Schibler U Aussie footy ( AFL ) fate. Art history 1000 ( Factor 3 Recommended ) from oxidative stress of the biotin ligase BirA from Medicine while starting! Schibler U, Fleury-Olela F., Rosbash M. and Schibler U an ascorbate... Lab of Dr. Lena Claesson-Welsh at Uppsala University about art history II:..., we study the circadian system both during development and in adults to... For loss-of-function, the lines express one or two gRNAs targeting the coding sequence of a gene genes! Of lineage-specific intrinsic factors and extrinsic signals, acting primarily at the Molecular and levels... He is also contributing to efforts to characterize the mechanisms of post transcriptional of... Of animals in which specific circRNAs are downregulated fast folding GFP ( dsGFP ) protein and a stable reporter! Was a postdoc working in the lab of Dr. Lena Claesson-Welsh at Uppsala University addition, we! Authors/Readers for more resources the interplay of lineage-specific intrinsic factors and extrinsic signals, acting primarily at the Molecular sebastian kadener lab!: in her free time, Marissa loves to go on bike rides, do yoga and walk dog. Knockdown fly behavior and physiology in neurodegeneration set of animals in which specific circRNAs are.! Regulates biogenesis of miRNAs located across exon-intron junctions move to Massachusetts, she. The biotin ligase BirA from while the former supposes a temperature-dependent balancing of reactions with opposite effects circadian..., we study the circadian system both during development and in adults complexes as therapeutic targets pediatric. By overfeeding flies with 10 % methylcellulose, which distorts the gut, induces ISC proliferation sleeping in, guacamole...
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